The Faculty of Life Sciences and Biotechnology announces International Workshop on “International workshop on CRISPR/ Cas9 &/13 for Gene Editing and Nucleic Acid based Molecular Diagnostic” 6-9^th of January 2025.

About the workshop

Our 5 days workshop will provide hand-on training in knocking out of target genes in mammalian cells using highly efficient CRISPR/Cas9/13 system. Cas13 will be used for the Nucleic Acid based Diagnostic. Attendees will learn designing of CRISPR/Cas targets using bioinformatics tools, construction of sgRNA, generation and validation of gene knockout cell lines and nucleic acid based molecular diagnostic.

Registration Fee:

Students (pursuing Ph.D. and M.Sc.)/Research Scholars and Post-Doctoral Fellow: Rs 10,000

Faculty/Scientist: Rs 12,000

Industry Delegates: Rs 12,000

South Asian University students are considered for 50% discount in registration fees.

Registration is open to Faculty/Research Scholars/Students from any SAARC country with a Bachelor degree in any field of Biology/Medicine. Registration fee covers access to all days of workshop and Lunch. Accommodation is not included in the registration fee.

The registration &payment of fee can be done online. Please note that the registration fee is non-refundable if you are selected for attending the workshop.

TA/DA may be reimbursed to the candidates of SAARC countries other than India (maximum INR 6,000 up to 5 candidates)

Tentative workshop program

Day 1# (06/01/2025)  From (9:00 AM-5:00 PM)

Lecture on: CRIPSR cas13a and specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) detection system – Design of Recombinase Polymerase Amplification (RPA) primers and cas13a guideRNA, gRNA Designing (participant need to bring their Laptop)CRISPR/Cas9 and Designing, Cloning and Mammalian Cell Culture for CRISPR/Cas9

Day 2# (07/01/2025)  From (9:00 AM-5:00 PM)

Lecture on: Introduction of CRISPR/Cas9 past and Present, Clonning CRISPR/Cas9, Transfection of Mammalian cells with CRISPR/Cas9 plasmid with GFP-Cas9 empty vector and GFP Cas9 plasmid of interest to HEK293 mammalian cells,  Recombinase Polymerase Amplification (RPA) – Performance and Validation of amplicons on agarose gel  and  In-Vitro Transcription (IVT) reaction setup and overnight incubation

Day 3# (08/01/2025)  From (9:00 AM-5:00 PM)

guideRNA purification for Cas13a,  cas13a detection assay – Microplate kinetic detection, Lateral flow assay demo,  Genomic DNA isolation, PCR, Heteroduplex formation and Surveyor assay for CRISPR/Cas9, DNA page running, Detection (GFP clone in fluorescence microscope)CRISPR/Cas9 and  Cell Lysate Preparation, Protein Estimation, Western Blotting, Blocking, Immunoblotting assay etc. For validation of CRISPR/Cas9 Knockout of gene in HEK293 cells.

Day 4# (09/01/2025)  From (9:00 AM-5:00 PM)

Secondary antibody incuabation and development of blot with target gene for the validation of CRISPR/Cas9 mediated gene knockdown in cell lines, and Results discussion and Question and Answer session.

Note: All Participants are allowed to performed each of these experiments with hands on training program